Furthermore, their different capacities to utilize light energy mean that sun and shade leaves do not require the same level of photoprotection at a given light.
Although CEF activation is involved in ATP synthesis and photoprotective functions, the specific role of CEF under different light intensities in each leaf type is unclear.
This work was to test whether the role of CEF can be regulated flexibly in response to incident light in the sun and shade leaves. Under stronger light, however, the rise in CEF was very large for sun leaves but only slight for shade leaves. Furthermore, these results support an earlier conclusion by Miyake et al. Therefore, our estimates of CEF in sun and shade leaves are reliable.
Such a change in energy demand necessitates a flexible mechanism to add ATP synthesis and then balance that ratio. Because the Rubisco content was lower in the shade leaves, the rate of CO 2 assimilation was restricted Table 2 and Yamori et al. This hypothesis is supported by research with mutants that lack key enzymes for the CEF pathway.
For tobacco plants, light saturating point LSP of CO 2 assimilation is lower in the shade leaves than the sun leaves Huang et al. Our results strongly suggest that the main function of CEF is flexibly changed according to lighting conditions in sun and shade leaves of tobacco. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Amthor, J. From sunlight to phytomass: on the potential efficiency of converting solar radiation to phyto-energy.
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Low light e. Enhancing photosynthetic capacity of plants is a promising approach to increase crop productivity 30 , Improving the efficient photosynthesis at low light by through conventional breeding and genetic engineering would be of great importance for producing tolerable cultivars under low light. Our results indicate that NDH-dependent Cyclic electron transport plays an important role for photosynthesis and plant growth at low light intensity and at low temperature.
In the northern area of Japan, cool weather damage in summer is always caused not only by low temperature but also by a shortage of sunshine. However, little is known whether plants are potentially able to acclimate to such combined stress conditions of low temperature and low light Therefore, based on the present discovery, enhancing NDH-dependent Cyclic electron transport could contribute to improvements of photosynthesis and plant growth under such cool weather damage in summer.
Hitomebore were used. The plants were grown hydroponically in an environmentally controlled growth chamber as described Measurements of gas exchange, chlorophyll fluorescence and P redox state were performed simultaneously with a GFS and a Dual-PAM measuring system Walz, Effeltrich, Germany in uppermost, fully expanded new leaves of to days-old plants as described The frozen leaf samples were ground in liquid nitrogen and homogenized in an extraction buffer.
Contents of leaf nitrogen, chlorophyll and Rubisco were quantified The amount of Rubisco large subunit was determined spectrophotometrically by formamide extraction of the Coomasie Brilliant Blue Rstained bands corresponding to the large and small subunits of Rubisco. A dilution series of WT proteins was loaded on gels to estimate the protein level in the mutant. Equal amounts of chlorophyll were loaded in each lane of a gel.
How to cite this article : Yamori, W. Photosystem I cyclic electron flow via chloroplast NADH dehydrogenase-like complex performs a physiological role for photosynthesis at low light.
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It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. The pathway of CET. Does cyclic electron transport occur in C 3 plants? Steady-state measurements of photosynthesis. The role of CET in C 3 plants. Regulation of cyclic electron transport. Cyclic electron transport in C 3 plants: fact or artefact?
Johnson Giles N. School of Biological Sciences, University of Manchester, 3. E-mail: giles. Oxford Academic. Google Scholar. Cite Cite Giles N. Select Format Select format. Permissions Icon Permissions. Abstract The phenomenon of cyclic electron transport was first characterized in higher plant chloroplasts 50 years ago, yet there is still a debate about whether or not this is a physiological process.
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